FastQCFastQC Report
Wed 14 Oct 2020
HHL37AFX2_n01_AH10675-3H-Hop1MLChip-rep.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL37AFX2_n01_AH10675-3H-Hop1MLChip-rep.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6803205
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC2302143.3839050859117132TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA590900.062.7202459
AGAGCAC614200.060.4825678
CGGAAGA685900.054.1779824
TCGGAAG685950.054.1423153
GAAGAGC695500.053.503166
AAGAGCA701500.053.141687
GATCGGA699150.052.4766851
ATCGGAA711400.052.1755872
GGAAGAG719350.051.70915
AGGGGGG213100.023.69904970-74
GCCGTCT293150.023.5488245-49
TGCCGTC311850.023.23867645-49
AAGGGGG187600.022.80079870-74
ATGCCGT322350.022.64364845-49
GTATGCC321850.022.6202645-49
CCGTCTT312100.022.27230550-54
TATGCCG328450.022.26725445-49
CGTATGC327550.022.2310540-44
CTGCTTG282100.022.00406855-59
TCGTATG336050.021.80249840-44
Produced by FastQC (version 0.11.5)