FastQCFastQC Report
Wed 14 Oct 2020
HHL37AFX2_n01_AH10515-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL37AFX2_n01_AH10515-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9094295
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT226410.24895827549029365TruSeq Adapter, Index 27 (97% over 44bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACC43950.016.165821145
ATGCCGG41850.015.764367145
CACACGG28500.011.192792145
ATCTCGG37650.011.168468145
GCACACC39700.010.4091425145
ATCGCGG27350.010.338032145
TGCCGTG42650.09.519181145
AAGAGCC86300.09.32487145
TTTATCG173050.08.92357145
TGCCGGG23950.08.778556145
AAGGGGG75350.08.65944170-74
TCTCGTT89400.08.433861145
GTATGCC155500.07.739418145
CTTTATG102350.07.6500897145
GCCGTCG45600.07.4724607145
GCCGGCG24305.7480065E-107.160372145
CCAGTCA621850.07.158356145
AAAGGGG95350.07.147229770-74
CAGTCAC589250.07.136069145
AGGGGGG72650.07.045303370-74
Produced by FastQC (version 0.11.5)