FastQCFastQC Report
Wed 14 Oct 2020
HHL37AFX2_n01_AH10515-Red1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL37AFX2_n01_AH10515-Red1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7665756
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA344750.019.4942689
AGAGCAC365100.018.4076928
TCGGAAG436000.015.4356923
CGGAAGA437900.015.401844
GAAGAGC452700.014.9623926
GATCGGA449200.014.7932931
AAGAGCA464000.014.7185297
ATCGGAA467350.014.462522
GGAAGAG480200.014.1055295
AGGGGGG69700.013.70926470-74
AAGGGGG71750.012.77193370-74
AAAGGGG83800.010.83157970-74
CACAGTG155050.08.9589130-34
CACACAG158800.08.71995630-34
GTCACAC160600.08.4597125-29
ACACAGT167400.08.4278930-34
GTATGCC51300.07.77278745-49
CAGTGAT182000.07.727905335-39
CAGTCAC183950.07.511982425-29
AGTCACA187300.07.400849325-29
Produced by FastQC (version 0.11.5)