FastQCFastQC Report
Wed 14 Oct 2020
HHL37AFX2_n01_AH10514-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL37AFX2_n01_AH10514-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10138077
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT834440.8230752242264485TruSeq Adapter, Index 25 (97% over 44bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG32800.018.56679145
GAGCACC49150.015.930581145
GCCGTCG42350.013.181618145
AAGGGGG120850.012.80203970-74
GCACACC43900.011.560187145
AAAGGGG139350.011.41460870-74
AGGGGGG126400.010.80600670-74
CACACGG34050.09.7942705145
CTGCTTG234100.09.6623955-59
GCTTGAA256900.09.23944660-64
GCCGTCT267350.09.23625350-54
CCAGTCC38150.09.121748145
TGCTTGA254700.08.95490860-64
TGCCGTG42400.08.891375145
CCGTCTT290000.08.58486850-54
AAGAGCC95450.08.582897145
TGCCGTC298500.08.42784550-54
CTTCTGC296150.08.11282655-59
CGTCTTC317500.07.914366750-54
ATGCCGT324650.07.88745145-49
Produced by FastQC (version 0.11.5)