FastQCFastQC Report
Wed 14 Oct 2020
HHL37AFX2_n01_AH10514-Red1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL37AFX2_n01_AH10514-Red1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9322397
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC488200.5236850565364252TruSeq Adapter, Index 4 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG84900.015.79771870-74
GAGCACA585600.015.6610119
AGAGCAC619100.014.801878
AAGGGGG84700.013.55820170-74
GCCGTCT103300.013.30663845-49
TGCCGTC119200.012.89406645-49
GAAGAGC734100.012.5370446
AAGAGCA743250.012.5342567
CGGAAGA758200.012.2820254
TCGGAAG763150.012.1073263
ATGCCGT133600.012.06864845-49
TATGCCG140950.011.80965245-49
GGAAGAG790300.011.7648065
GTATGCC142300.011.75875245-49
GATCGGA798050.011.4185621
ATCGGAA814750.011.3406642
CGTATGC150300.011.1328740-44
CCGTCTT128400.011.07807550-54
GAGCACC39500.010.46184145
TCGTATG164550.010.31856540-44
Produced by FastQC (version 0.11.5)