FastQCFastQC Report
Wed 14 Oct 2020
HHL37AFX2_n01_AH10513-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL37AFX2_n01_AH10513-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9089401
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT151530.1667106556306626TruSeq Adapter, Index 7 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG36450.022.475594145
GAGCACC44500.014.499741145
TGCCGTG41950.013.652915145
CACACGG33000.012.302811145
GCCGTCG46500.012.005161145
GATATCG97300.011.996189145
GCACACC38550.011.09578145
ATCTCGG35750.010.545266145
TCTCGTT86950.010.005571145
GGATATG91100.09.629355145
AGGGGGG69800.08.4193331
CTCGTAT269600.07.6639943145
GTATGCC221850.07.3201303145
CGAGTGG496950.07.148478145
TCACGAG578450.07.056231145
AAGAGCC82550.07.025919145
TATCTCG311250.06.9878263145
CGTATGC236200.06.967488145
TATGCCG213450.06.928899145
AGTGGAT495550.06.8029256145
Produced by FastQC (version 0.11.5)