FastQCFastQC Report
Wed 14 Oct 2020
HHL37AFX2_n01_AH10513-Red1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL37AFX2_n01_AH10513-Red1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7581308
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT2689273.547237495165742TruSeq Adapter, Index 19 (97% over 40bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA896100.051.811559
AGAGCAC930050.049.9358448
GAAGAGC1033250.045.1508456
AAGAGCA1033700.045.0970547
CGGAAGA1056000.044.2674144
TCGGAAG1065650.043.8325233
GGAAGAG1076800.043.378655
GATCGGA1070500.043.2620661
ATCGGAA1110500.042.1283872
GCCGTCT358450.024.6267950-54
TGCCGTC378400.024.02581450-54
AAGGGGG246700.023.72158670-74
GTATGCC399800.023.30193945-49
TATGCCG400550.023.27278945-49
CCGTCTT381450.023.2711350-54
ATGCCGT396300.023.18210245-49
AAAGGGG258050.023.06593170-74
CGTATGC404300.023.01030345-49
CTGCTTG342450.022.74153155-59
CTCGTAT402050.022.15090440-44
Produced by FastQC (version 0.11.5)