FastQCFastQC Report
Wed 14 Oct 2020
HHL37AFX2_n01_AH10512-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL37AFX2_n01_AH10512-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8898160
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT189220.21265070531435712TruSeq Adapter, Index 22 (97% over 40bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG48150.015.508577145
CACACGG27850.014.317544145
CGTAATG87600.013.655602145
GAGCACC41500.013.2768955145
ATCTCGG49600.013.0088625145
CCAGTCC39450.011.761535145
ATCGCGG41400.011.7329035145
GTAATCG141000.011.363293145
GCACACC37100.010.94322145
TAATCGG57050.09.022642145
GCCGTCG46800.08.984898145
TGCCGGG30400.08.585388145
AAGAGCC79100.07.7906456145
TCTCGTT93550.07.7497416145
GTCACCC35900.07.6739717145
AAGGGGG79550.07.509621670-74
TGCCGTG40950.07.2587357145
CGTATGG60950.07.1368833145
TCGCGGG14305.2823736E-57.097788145
GCGGGGG26706.202754E-106.78828145
Produced by FastQC (version 0.11.5)