FastQCFastQC Report
Wed 14 Oct 2020
HHL37AFX2_n01_AH10512-Red1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL37AFX2_n01_AH10512-Red1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7433546
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT421100.5664860350632122TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA398100.018.9214959
AGAGCAC424100.017.8469628
GTCCGCC20650.015.447731145
GCCGTCT69950.015.19421850-54
GAAGAGC512100.015.1239576
TGCCGTC80500.014.95010750-54
CGGAAGA524250.014.897944
AAGAGCA517100.014.8755467
TCGGAAG525700.014.7326973
CGTATGC93250.014.16548945-49
TATGCCG94300.014.10001945-49
GATCGGA544300.014.0876181
GTATGCC95750.013.97735545-49
ATCGGAA554400.013.9441392
GGAAGAG556450.013.9315855
ATGCCGT92150.013.79959445-49
CATCTCG102400.013.62188540-44
AAGGGGG70800.012.94332770-74
CTCGTAT101600.012.77295740-44
CCGCACA121100.012.75168435-39
Produced by FastQC (version 0.11.5)