FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_9_input_nlp5_5_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_9_input_nlp5_5_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22583170
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT338100.14971325991878023TruSeq Adapter, Index 18 (97% over 40bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC74450.037.3797446
TATGCCG76250.036.269848
GTATGCC78100.035.72402647
TCCGCAC93000.032.44196735
GCCGTCT78200.031.91836751
CCGCACA93200.031.77175336
CGTCCGC96450.031.35354833
GTCCGCA97850.030.83368934
CGCACAT96800.029.83393537
CTCGTAT93050.029.53089144
TCGTATG96400.029.04959545
ACGTCCG110050.027.51061432
ATGCCGT104150.026.55362549
TCTCGTA101900.026.24678443
ATCTCGT115100.023.44895642
CATCTCG118650.023.01284641
GCACATC123600.022.91225238
TCACGTC136550.022.37604330
CACGTCC136650.022.18048131
TGGGGGG34250.022.1753481
Produced by FastQC (version 0.11.2)