FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_8_chip_tga1_180_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_8_chip_tga1_180_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14240430
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC1103750.7750819322169344TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATG256700.1802614106456055TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG180400.051.51817346
CGTATGC181800.051.29419344
ATGCCGT180500.050.88950347
GTATGCC187650.049.88199245
GCCGTCT171200.049.42203549
TGCCGTC180600.049.36934748
CTCGTAT179550.048.4109542
CGTAGAG212000.048.2914933
GTCACGT212400.048.11578429
ACGTAGA215200.047.70318232
TCGTATG197650.047.3615643
TCACGTA217500.047.1814830
AGTCACG217650.047.0700528
CACGTAG218200.046.99820731
TCTCGTA188050.045.86855741
CCGTCTT195650.043.4963550
ATCTCGT201200.042.54055840
GATCTCG204100.042.0039839
CAGTCAC248200.041.4034327
CTCCAGT254100.041.3771524
Produced by FastQC (version 0.11.2)