FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_7_chip_tga1_30_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_7_chip_tga1_30_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13255406
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC238310.1797832522066846TruSeq Adapter, Index 16 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCC123450.051.0334733
TATGCCG59750.048.15670446
CCGTCCA129700.047.3332834
CGTATGC59550.046.84890444
TCACCCG137300.046.19072730
ATGCCGT57900.045.9495147
CACCCGT138700.045.75007231
CGTCCAT126250.044.02583335
AGTCACC146700.043.66357428
GTCACCC145950.043.476829
GTATGCC67400.043.2619745
TGCCGTC58300.042.69268448
ACCCGTC150950.041.99159232
ACTCCAG160400.041.04526523
CAGTCAC157350.040.8193427
CTCCAGT161550.040.51445824
GCCGTCT52700.040.3873549
TCCAGTC166200.039.3813725
CCAGTCA163250.039.06506726
ACGTCTG174100.038.15082615
Produced by FastQC (version 0.11.2)