FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_6_chip_tga1_10_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_6_chip_tga1_10_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11956531
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT937200.7838393928807611TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA231190.19335875932576096TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC143750.050.62774746
TATGCCG143050.050.43690548
GTATGCC148100.049.236947
GCCGTCT135700.049.11853451
ATGCCGT145850.048.7725649
TGCCGTC144500.047.82288750
CTCGTAT148950.047.77899644
TCGTATG157750.046.5788445
TCTCGTA156100.045.6165843
ACAGTCA179450.044.5492232
CACAGTC180800.044.15794431
CCGTCTT155200.043.30742652
GTCACAG183950.043.2874329
CAGTCAA186850.042.8412733
ATCTCGT168300.042.8076542
TCACAGT187900.042.5078430
CAGTCAC188350.042.46469527
AGTCAAC191450.041.68393734
CTCCAGT195950.041.42278724
ACTCCAG197000.041.27342223
Produced by FastQC (version 0.11.2)