FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_5_chip_tga1_5_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_5_chip_tga1_5_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10060488
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC638270.6344324450265236TruSeq Adapter, Index 11 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG102390.10177438708738583TruSeq Adapter, Index 11 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG94600.055.80205546
CGTATGC97650.054.739744
ATGCCGT95700.053.69830747
GTATGCC100050.053.5673645
CACGGCT110350.053.19046431
GTCACGG110250.052.9831629
TGCCGTC94400.052.65859648
GCCGTCT91300.052.1457549
AGTCACG111900.052.1445328
ACGGCTA117400.050.2648832
CTCGTAT103600.049.9749942
CGGCTAC118300.049.7939733
ACGTCTG122200.049.25966315
GCACACG122900.049.11610411
TCGTATG110700.048.41578343
ACACGTC125850.048.0482113
CGTCTGA126050.047.69957416
ACTCCAG124850.047.65773423
CACACGT127400.047.4361712
CTCCAGT126050.047.0927324
Produced by FastQC (version 0.11.2)