FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_4_chip_nlp7_180_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_4_chip_nlp7_180_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11635140
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTAGC118000.043.63308331
TATGCCG30100.041.28584746
TCACTAG125350.041.13046630
ACTAGCT126000.040.78026632
TAGCTTA127350.040.3474534
AGTCACT129650.039.9570228
CTCCAGT131750.039.2375624
GTCACTA132200.039.21023629
ACGTCTG133950.038.84790815
ACTCCAG133550.038.70854623
CAGTCAC135050.038.22924427
CGTCTGA137500.037.94705216
CGTATGC29300.037.7523444
CCAGTCA137750.037.40337426
ACACGTC141650.037.0064813
TCCAGTC140250.036.96030425
CACACGT141100.036.9275312
GCACACG140650.036.9212811
GTATGCC35450.036.53553845
GCTTATC132150.036.49847436
Produced by FastQC (version 0.11.2)