FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_3_chip_nlp7_30_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_3_chip_nlp7_30_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11963799
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC591490.4943998139721338TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG91700.052.75666446
CGTATGC94700.051.34263644
GTATGCC96050.050.62185345
GCCGTCT93450.050.3109549
ATGCCGT97450.049.46664447
AGTCACG100200.049.1146228
CTCGTAT99850.046.02997242
TGCCGTC103950.045.9701448
CGATCAG107400.045.8854833
GTCACGA107750.045.6695529
TCGTATG110650.044.07007243
TCTCGTA106200.042.81697541
CAGTCAC117650.042.03848327
CCGTCTT114450.041.4160550
CCAGTCA119400.041.27577226
CACGATC120800.040.7363931
TCACGAT122300.040.43725630
ACTCCAG123800.040.2601423
CTCCAGT124050.040.2354324
GATCTCG111200.040.19920339
Produced by FastQC (version 0.11.2)