FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_2_chip_nlp7_10_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_2_chip_nlp7_10_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12573715
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC621330.49414989921435315TruSeq Adapter, Index 6 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG129340.10286538226769097TruSeq Adapter, Index 6 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG91850.050.1175246
CGTATGC93700.049.38775644
GCCGTCT84650.048.46980349
CACGCCA110650.048.0167231
ATGCCGT94350.047.67894447
GTATGCC97750.047.4131345
AGTCACG113100.047.0733428
CTCGTAT101400.045.8107442
GTCACGC117450.045.47419729
TCACGCC117550.045.37632430
ACGCCAA119300.044.65451432
CGCCAAT121100.044.01933333
TGCCGTC99350.043.94057548
GCCAATA124100.042.89863234
TCGTATG109200.042.70018443
TCTCGTA111300.042.58511441
CAGTCAC130450.040.78586227
CCAGTCA132850.039.94349726
TATCTCG125350.039.7389739
TCCAGTC135700.039.5940625
Produced by FastQC (version 0.11.2)