FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_28_nlp7_dam_nlp7_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_28_nlp7_dam_nlp7_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9895865
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCGA3308850.069.30736
GCGGCCG3312850.069.203545
GAGGATC3275350.069.1892611
GGTCGCG3312100.069.069411
AGGATCT816650.069.05858612
GGCCGAG3322900.069.012147
AGGATCA1040000.068.9848212
CGCGGCC3325100.068.919124
CGAGGAT3291450.068.917810
AGGATCG650700.068.8655712
CCGAGGA3294150.068.852819
GCCGAGG3339350.068.686858
GTCGCGG3336400.068.627312
TCGCGGC3348800.068.356143
GGATCTA77800.067.7802413
GGATCGT184050.067.6646113
GGATCCG221800.067.60187513
AGGATCC797500.067.5752612
GGATCTT239400.067.5285313
GGATCAT288450.067.3516713
Produced by FastQC (version 0.11.2)