FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_27_nlp7_dam_gr_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_27_nlp7_dam_gr_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9635793
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGCGGCCGAGGATCCGCCTAGGCTGTCCCGAGTGTGAGCGAGGTGTG405530.4208579408046645No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCT379270.3936053835942719No Hit
GGTCGCGGCCGAGGATCGGGTTGCGGTTTAAGTTCTTATACTCAATCATA172380.17889549931178472No Hit
GGTCGCGGCCGAGGATCTTAAAGGCGTAAGAATTGTATCCTTGTTAGAAG170730.17718313375972272No Hit
GGTCGCGGCCGAGGATCTTAAAAGCCTAAGTAGTGTTTCCTTGTTAGAAG170350.17678877078409636No Hit
GGTCGCGGCCGAGGATCGGGTTGCGGTTTAAGTTGTTATACTCAATCATA164730.1709563499340428No Hit
GGTCGCGGCCGAGGATCAAGTCATATTCGACTCCAAAACACTAACCAACC110350.11452093252729692No Hit
GGTCGCGGCCGAGGATCCGGTTGCGGTTTAAGTTCTTATACTCAATCATA98930.10266928731241944No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG2914550.069.7776641
CGCGGCC2937250.069.3089754
GTCGCGG2935600.069.288322
GCGGCCG2937950.069.236485
TCGCGGC2937950.069.201943
AGGATCT1000200.069.1731612
CGGCCGA2945100.069.142056
AGGATCA900800.069.0408612
GAGGATC2928500.069.0046511
AGGATCG300300.068.9710512
GGCCGAG2946100.068.951127
GGATCTA158750.068.9267813
CGAGGAT2940850.068.7862610
CCGAGGA2940600.068.761179
GCCGAGG2956600.068.755958
GGATCTC235700.068.7377413
GGATCAA341200.068.69260413
GGATCCG166800.068.6841413
GGATCTT453900.068.61256413
GGATCCA303900.068.5455113
Produced by FastQC (version 0.11.2)