FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_26_col_dam_nlp7_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_26_col_dam_nlp7_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13902025
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGCGGCCGAGGATCCGCCTAGGCTGTCCCGAGTGTGAGCGAGGTGTG356000.2560778016152323No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCT350840.25236611213114635No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG3456900.069.327651
GCGGCCG3462300.069.093655
CGGCCGA3473350.069.019926
CGCGGCC3482350.068.860634
GGCCGAG3469300.068.7159657
AGGATCT952300.068.6379312
GTCGCGG3493400.068.6242752
GAGGATC3415850.068.5721911
AGGATCA1112550.068.5391412
TCGCGGC3500050.068.4714053
GCCGAGG3485250.068.360338
CCGAGGA3429000.068.359229
CGAGGAT3431700.068.3533610
AGGATCG494550.067.8955212
GGATCGT146500.067.04516613
GGATCTT312050.067.0002513
GGATCTA130050.066.9426213
GGATCAT326500.066.84289613
GGATCTC269350.066.8131913
GGATCCA324300.066.5626313
Produced by FastQC (version 0.11.2)