FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_25_col_dam_gr_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_25_col_dam_gr_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9614508
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCT863990.8986315264390023No Hit
GGTCGCGGCCGAGGATCCGCCTAGGCTGTCCCGAGTGTGAGCGAGGTGTG833950.867387077945122No Hit
GGTCGCGGCCGAGGATCGGGTTGCGGTTTAAGTTCTTATACTCAATCATA305480.3177281666414964No Hit
GGTCGCGGCCGAGGATCTTAAAAGCCTAAGTAGTGTTTCCTTGTTAGAAG283710.29508530233684344No Hit
GGTCGCGGCCGAGGATCGGGTTGCGGTTTAAGTTGTTATACTCAATCATA258870.2692493469244604No Hit
GGTCGCGGCCGAGGATCAAGTCATATTCGACTCCAAAACACTAACCAACC216620.22530534063729524No Hit
GGTCGCGGCCGAGGATCTTAAAGGCGTAAGAATTGTATCCTTGTTAGAAG208030.21637092610459113No Hit
GGTCGCGGCCGAGGATCTTAAAGGCGTAAGAATTGTATCCTTGTTAAAAG142720.14844233319063233No Hit
GGTCGCGGCCGAGGATCCATTTTGCCGACTTCCCTTGCCTACATTGTTCC141670.14735023362609923No Hit
GGTCGCGGCCGAGGATCTTATAAGCCTAAGTAGTGTTTCCTTGTTAGAAC131510.13678287022071228No Hit
GGTCGCGGCCGAGGATCTTATAAGCCTAAGTAGTGTTTCCTTGTTAGAAG97670.10158606139804555No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG2890600.069.803241
CGCGGCC2918450.069.167234
GTCGCGG2917450.069.137322
TCGCGGC2916850.069.131993
GCGGCCG2915150.069.096695
CGGCCGA2925400.068.976596
GGCCGAG2921500.068.744927
GCCGAGG2926350.068.587948
CCGAGGA2903350.068.5190359
CGAGGAT2905600.068.4635710
GAGGATC2908250.068.3674911
AGGATCT973200.068.2790912
AGGATCA813950.068.1047112
AGGATCG370250.067.6112212
GGATCGG162150.067.0269313
GGATCGT84750.066.9280513
GGATCTA140250.066.6650913
GGATCTT496900.066.5257513
GGATCAA316550.066.21393613
GGATCAT241150.065.9494913
Produced by FastQC (version 0.11.2)