FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_23_nlp7_dam_gr_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_23_nlp7_dam_gr_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14488023
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCT462030.31890479467074284No Hit
GGTCGCGGCCGAGGATCCGCCTAGGCTGTCCCGAGTGTGAGCGAGGTGTG440740.30420989806545723No Hit
GGTCGCGGCCGAGGATCGGGTTGCGGTTTAAGTTCTTATACTCAATCATA278900.19250383575454014No Hit
GGTCGCGGCCGAGGATCTTAAAAGCCTAAGTAGTGTTTCCTTGTTAGAAG264340.18245415540822926No Hit
GGTCGCGGCCGAGGATCTTAAAGGCGTAAGAATTGTATCCTTGTTAGAAG257000.1773879017171632No Hit
GGTCGCGGCCGAGGATCGGGTTGCGGTTTAAGTTGTTATACTCAATCATA251830.17381943692386465No Hit
GGTCGCGGCCGAGGATCAAGTCATATTCGACTCCAAAACACTAACCAACC187150.12917566461621438No Hit
GGTCGCGGCCGAGGATCCGGTTGCGGTTTAAGTTCTTATACTCAATCATA165320.11410804634973315No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG4310750.069.725261
CGCGGCC4322600.069.333684
GTCGCGG4329350.069.290482
TCGCGGC4326250.069.269513
GCGGCCG4303200.069.259985
CGGCCGA4319900.069.151016
GGCCGAG4279150.068.970267
AGGATCT1502500.068.9474312
AGGATCA1297250.068.85052512
GCCGAGG4284850.068.840138
CCGAGGA4258900.068.821659
GAGGATC4261300.068.77901511
CGAGGAT4268600.068.7128110
AGGATCG443050.068.6434612
GGATCTC336400.068.6026613
GGATCTT711450.068.5597813
GGATCTA237900.068.5299513
GGATCAA504700.068.4261313
GGATCTG226450.068.4254213
GGATCAG191600.068.4154913
Produced by FastQC (version 0.11.2)