FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_22_col_dam_nlp7_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_22_col_dam_nlp7_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14858667
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCT362590.24402592776323742No Hit
GGTCGCGGCCGAGGATCCGCCTAGGCTGTCCCGAGTGTGAGCGAGGTGTG338220.2276247256904001No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG4015500.069.316781
GCGGCCG3984150.069.129955
CGGCCGA3997550.069.115326
CGCGGCC4037200.068.8950354
GGCCGAG3949050.068.7865147
GTCGCGG4046400.068.6899642
GAGGATC3905100.068.6361511
GCCGAGG3959700.068.502538
CCGAGGA3919350.068.478349
TCGCGGC4058500.068.478273
AGGATCT1105150.068.4470412
AGGATCA1280850.068.4259112
CGAGGAT3923750.068.4015510
AGGATCG562400.068.156612
GGATCGT172150.067.2010913
GGATCTA144900.067.0154713
GGATCAT380450.066.8954413
GGATCCA374950.066.5981213
GGATCTT371650.066.5962813
GGATCTC318650.066.5266813
Produced by FastQC (version 0.11.2)