FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_21_col_dam_gr_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_21_col_dam_gr_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12823522
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCT1061620.8278692858327065No Hit
GGTCGCGGCCGAGGATCCGCCTAGGCTGTCCCGAGTGTGAGCGAGGTGTG1044320.814378452347179No Hit
GGTCGCGGCCGAGGATCCATTTTGCCGACTTCCCTTGCCTACATTGTTCC389720.30391026739767746No Hit
GGTCGCGGCCGAGGATCGGGTTGCGGTTTAAGTTCTTATACTCAATCATA362260.2824964935530192No Hit
GGTCGCGGCCGAGGATCTTAAAAGCCTAAGTAGTGTTTCCTTGTTAGAAG360350.2810070431508598No Hit
GGTCGCGGCCGAGGATCGGGTTGCGGTTTAAGTTGTTATACTCAATCATA310870.24242169974832187No Hit
GGTCGCGGCCGAGGATCAAGTCATATTCGACTCCAAAACACTAACCAACC276750.21581434492021773No Hit
GGTCGCGGCCGAGGATCTTAAAGGCGTAAGAATTGTATCCTTGTTAGAAG252600.19698176522799277No Hit
GGTCGCGGCCGAGGATCCGGTTAAAATTCCGGAACCGGGACGTGGCGGTT213830.16674826151505023No Hit
GGTCGCGGCCGAGGATCTTAAAGGCGTAAGAATTGTATCCTTGTTAAAAG176010.137255583918365No Hit
GGTCGCGGCCGAGGATCTTATAAGCCTAAGTAGTGTTTCCTTGTTAGAAC168580.1314615438722685No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG3904900.069.789331
CGCGGCC3946750.069.073674
GTCGCGG3944300.069.050032
TCGCGGC3945650.069.028183
GCGGCCG3927300.069.026385
CGGCCGA3946900.068.850286
GGCCGAG3920750.068.599047
CCGAGGA3916650.068.388529
GCCGAGG3929550.068.364388
CGAGGAT3922650.068.27231610
GAGGATC3922750.068.2027811
AGGATCT1294650.068.0191712
AGGATCA1089700.067.9156212
AGGATCG498500.066.9754312
GGATCGG201600.066.7935813
GGATCTT661900.066.1914813
GGATCTA179800.066.1727813
GGATCCG335800.066.09000413
GGATCAA417900.065.9764513
GGATCTC290250.065.8038813
Produced by FastQC (version 0.11.2)