FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_20_c_r_ev_nlp7_h3k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_20_c_r_ev_nlp7_h3k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12676876
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCGA20900.038.1745876
GGTCGCG20850.037.6013221
GTCGCGG22550.035.5366442
CGTATGC19250.027.64006844
TATGCCG19300.027.38752246
GTATGCC21150.025.81916645
ATGCCGT21950.024.7199447
GCCGTCT23350.021.88885749
TGCCGTC24400.021.23394648
TCGCGGC45050.018.098683
GGCCGAG52650.016.2835877
CCGTCTT36900.014.1356950
TCTCGTA36000.013.22446341
CGAGGAT65350.012.74423310
CGCGGCC71750.012.5342114
GAGGATC67450.012.19181211
TCGTATG46050.012.16328443
ATCTCGT43750.012.00208240
GCCGAGG75350.011.5173028
AATCTCG48500.011.18733139
Produced by FastQC (version 0.11.2)