FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_1_chip_nlp7_5_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_1_chip_nlp7_5_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13750532
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG55550.050.16237646
CGTATGC56700.047.4769644
CACAGTG129150.045.9641233
GTCACAC132000.045.1558529
GTATGCC63750.044.47840545
ACACAGT139250.042.78124632
ATGCCGT57700.041.8030847
CTCCAGT146250.041.04188524
CAGTGAT137750.040.8853535
CAGTCAC147350.040.45311727
GCCGTCT46650.040.2233349
ACTCCAG151100.039.84077523
ACGTCTG151900.039.80948615
CACACAG149950.039.7741731
CCAGTCA150300.039.6358426
TCCAGTC153250.039.19133825
ACAGTGA149400.039.07834234
CGTCTGA156450.038.67408816
CACACGT160250.037.7548112
ACACGTC161850.037.33833713
Produced by FastQC (version 0.11.2)