FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_19_c_r_ev_nlp7_gr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_19_c_r_ev_nlp7_gr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17215656
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT29350.029.81992749
GTATGCC31050.029.4249545
CGTATGC31400.029.09696444
TGCCGTC30600.029.05959148
TATGCCG31650.028.53590646
ATGCCGT31600.028.25003447
CCGTCTT43000.020.67943650
TCTCGTA48450.017.77443541
ATCTCGT52050.016.34321640
GCTTGAA66400.012.80875559
TGCTTGA66900.012.66129558
TCGTATG79800.012.19597643
AGATCTC70750.011.97390638
GTCGCGG14100.011.4159032
CTCGTAT80500.011.39350742
AGTCACC87650.010.94243628
GGTCGCG14900.010.5704241
CTGCTTG81150.010.2225457
CGGCCGA16550.09.9373686
CCAAAAG103950.09.52888333