FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_17_c_r_ev_col_gr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_17_c_r_ev_col_gr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16620951
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGGG23900.021.53067839
GCATCGG27000.019.5772438
GTCACAT89900.013.31466429
TGAGCAT88550.013.04409535
GGGGTGT10900.012.84646252
CGGGGGT11800.012.16305642
GGGTGTA9500.011.78939359
ATGGGAG114850.010.7270591
GCATCGC19300.010.70121338
ACATGAG119650.010.64800332
TGGCGGG17650.010.11546748
TGCGGGG10550.09.95475649
TGGGAGA122850.09.9412172
GTGTGGG17050.09.85422455
GTATGCC12150.09.79561745
GAGAGCT128900.09.5832125
CACATGA134000.09.40292231
AGAGCTC130600.09.2441136
CATGAGC144550.08.95899333
ATGCCGT10250.08.87980647
Produced by FastQC (version 0.11.2)