FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_15_c_r_bzip1_col_gr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_15_c_r_bzip1_col_gr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16187146
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC22200.012.14191944
GTATGCC23850.011.595468545
GCCGTCT22300.010.51838549
TATGCCG26300.010.38214646
TGCCGTC24900.010.26360648
ATGCCGT22900.010.08957447
TAGCATC52300.09.23594636
GGTAGCA67100.08.65935534
CACGGTA90150.08.65610170
GTAGCAT63750.08.45547135
GTCACGG152700.08.24986270
ACGGTAG82850.08.1094670
CGGTAGC73400.07.963751333
CATCTCG48350.07.747394639
AGTCACG166150.07.7294869
ATCTCGT45700.07.660484340
CCGTCTT35950.07.59575350
TCTCGTA43550.07.154483341
TCACGGT143000.07.145457370
TCTGAAC469000.06.938943470
Produced by FastQC (version 0.11.2)