FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_13_c_r_tga1_col_gr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_13_c_r_tga1_col_gr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33128234
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC49250.014.50011346
GTATGCC51650.013.826611547
TATGCCG57050.012.88625748
ATGCCGT53900.012.60021849
CGTACGT84250.011.63255734
TGCCGTC60700.010.90027850
CCGTACG94200.010.21802433
GTACGTA97800.010.02108735
TACGTAA97050.09.91831836
AATCTCG93800.08.80781941
ATCTCGT95700.08.70609942
AGTCACC218150.08.40580470
TCTCGTA90050.08.31958643
CACCGTA146450.07.957158670
ACGTAAT131700.07.788000637
ACCGTAC135150.07.612625670
GTCACCG203400.07.60456770
TCACCGT184300.07.17744170
TCTGAAC701900.06.984994470
TCGTATG113150.06.682496545