FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_11_c_r_nlp7_nlp7_gr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_11_c_r_nlp7_nlp7_gr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33304218
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC63100.019.75009247
CGTATGC62750.019.580746
TATGCCG70250.017.78995948
GGCCTTA104850.015.32295536
GCCGTCT70550.014.14165651
TCGTATG95850.013.00145745
CTCGTAT99200.012.6682444
ATGCCGT101350.011.88191549
GCCTTAT133750.011.83008637
TCTCGTA110900.011.4272743
TATCTCG119600.011.23964141
GTGGCCT156450.010.42562534
CGTGGCC156850.010.39892933
CCGTCTT100850.010.274569552
ATCTCGT132250.010.2439142
CACGTGG197900.08.3831431
AGTCACG275450.08.19445370
GTCACGT232950.08.0219870
TCACGTG211650.07.755756430
CCTTATC216300.07.54172538
Produced by FastQC (version 0.11.2)