FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_10_input_tga1_5_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_10_input_tga1_5_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18485975
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT190140.10285635461478229TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC47150.035.9339246
GTATGCC50900.033.08137547
TATGCCG53750.031.32746148
ATGCCGT54250.031.16770449
TGCCGTC57350.029.42200350
TCTCGTA65600.024.33453843
TCGTATG72250.023.78920245
CTCGTAT68850.023.54024344
GCCGTCT71550.023.3380151
ATCTCGT73300.021.10916342
TGAAACG83600.020.93403635
CACGTGA84500.020.46126431
CGTGAAA88250.019.75146133
TCACGTG89400.019.33957730
CCGTCTT87500.019.203952
GAAACGA88600.019.12072836
GTCACGT92750.018.5654429
TGCTTGA96500.016.86542360
GCTTGAA99650.016.43771261
AGTCACG116300.014.98807728
Produced by FastQC (version 0.11.2)