FastQCFastQC Report
Sat 19 Feb 2022
HHJJYAFX3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJJYAFX3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184948926
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTC3350392118.11522874158242No Hit
GCAGTTCT3131096216.929518152487137No Hit
TGCACAAA2703493714.617514999789726No Hit
CTTTGCGG2504661813.542451173790543No Hit
TGCACCGT151017978.165387778461607No Hit
AAGTTGCA140588257.601463444021298No Hit
GCACAATG138883237.509274749721985No Hit
CTTGGTAC116293426.287866737869027No Hit
GGGGGGGG58602133.1685574643455894No Hit
CTTGCGGA2683540.14509627376803474No Hit
CTTTGCGA2076810.112291000813922No Hit
GCAGTCTA2047200.11069001828104695No Hit
TCTATCCG1957310.10582975756236618No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)