FastQCFastQC Report
Tue 18 Jun 2019
HHJHYBGXB_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJHYBGXB_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41636008
Sequences flagged as poor quality0
Sequence length55
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1166078128.006481793355402No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAGGCGCGCCGCTCTCTACGTTTA1464330.35169798218887843No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTTGTGCTCAAAACTCAAGGTTT923880.22189447172745286No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAGGGG773170.18569743766020988No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAGGG728740.1750263858148937No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCGTTTCCGCTTAACGGCGGTTT494460.11875778292673976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCC306150.045.9197546
AGCAGTG1502500.045.72
AAGCAGT1525900.045.072511
TAGGCGC199950.044.56855432
CTACGTT189500.044.41940747
TCTACGT192000.044.1012246
CTATCCG96550.043.992097
ATCAACG1585500.043.05098711
GTATCAA1585700.042.7613269
CATGGGG1238050.042.67373725
GCCGCTC209150.042.58833339
CGCAGAG1628750.042.3104716
GTACATG1575000.042.3037522
GAGTACA1598150.042.22001620
TACATGG1579750.042.12631223
AGTACAT1595900.042.10898621
AGAGTAC1607000.042.06125619
CAGAGTA1628900.041.90701718
ACATGGG1581300.041.8230824
CAACGCA1634600.041.79365513