FastQCFastQC Report
Tue 18 Jun 2019
HHJHYBGXB_n02_RNA_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJHYBGXB_n02_RNA_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86246981
Sequences flagged as poor quality0
Sequence length55
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1318120.15283085676935174No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1104200.1280276697453329No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT4000400.043.2651941
AGCAGTG4457750.038.7311442
AGTGGTA5186950.033.2144625
GCAGTGG5198000.033.159293
CAGTGGT5251950.032.792814
GTGGTAT5353950.032.1243066
TCAACGC5417100.031.6962212
CAACGCA5431050.031.65313
ATCAACG5462000.031.52472111
TGGTATC5522250.031.1329487
GTATCAA5548000.031.0747649
GGTATCA5545000.031.0329658
AACGCAG5552100.030.9522914
ACGCAGA5568750.030.86241515
TATCAAC5602500.030.808710
CGCAGAG5570250.030.75534216
GAGTACA5595500.030.31745320
GTACATG5510950.030.2778522
AGTACAT5647500.029.96256321
AGAGTAC5674300.029.943919
Produced by FastQC (version 0.11.5)