FastQCFastQC Report
Tue 18 Jun 2019
HHJHYBGXB_n02_RNA_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJHYBGXB_n02_RNA_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113782238
Sequences flagged as poor quality0
Sequence length55
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1407200.12367483930136793No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1207230.10610003997284709No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT5284750.043.373191
AGCAGTG5898100.038.7763942
AGTGGTA6871900.033.2328225
GCAGTGG6885350.033.1755753
CAGTGGT6964550.032.7717444
GTGGTAT7085900.032.181186
TCAACGC7171500.031.74577712
CAACGCA7195050.031.6662813
ATCAACG7226900.031.5680511
TGGTATC7310500.031.1833467
GTATCAA7350800.031.0875249
GGTATCA7341250.031.0819668
AACGCAG7351900.030.98687414
ACGCAGA7372200.030.90503515
TATCAAC7412450.030.85729810
CGCAGAG7368050.030.82520916
GAGTACA7407100.030.37461520
GTACATG7311450.030.32483722
AGAGTAC7514300.029.98137919
AGTACAT7485050.029.98089821
Produced by FastQC (version 0.11.5)