FastQCFastQC Report
Tue 18 Jun 2019
HHJHYBGXB_n02_RNA_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJHYBGXB_n02_RNA_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92778672
Sequences flagged as poor quality0
Sequence length55
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1328880.14323119434173404No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1141690.1230552211396171No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT4382000.043.3243871
AGCAGTG4881100.038.8256952
AGTGGTA5669150.033.3585365
GCAGTGG5683050.033.3032263
CAGTGGT5735800.032.9638254
GTGGTAT5842950.032.3249366
TCAACGC5920050.031.82039312
CAACGCA5930650.031.78727513
ATCAACG5969150.031.63430411
TGGTATC6043300.031.2361777
GTATCAA6061550.031.21099
GGTATCA6057400.031.1995538
AACGCAG6065750.031.08144414
ACGCAGA6082100.030.99540915
TATCAAC6120650.030.93221510
CGCAGAG6074750.030.9133116
GAGTACA6108550.030.46011220
GTACATG6021900.030.42000822
AGAGTAC6193350.030.08756619
AGTACAT6170000.030.08055521
Produced by FastQC (version 0.11.5)