FastQCFastQC Report
Tue 18 Jun 2019
HHJHYBGXB_n02_RNA_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJHYBGXB_n02_RNA_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91595596
Sequences flagged as poor quality0
Sequence length55
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1161270.12678229638900979No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1036200.11312770976456116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT4289650.043.2691421
AGCAGTG4780300.038.707062
GCAGTGG5556350.033.242263
AGTGGTA5560350.033.208255
CAGTGGT5621400.032.823074
GTGGTAT5731800.032.1740846
TCAACGC5807200.031.72990212
CAACGCA5821750.031.69052913
ATCAACG5858650.031.52499411
TGGTATC5913500.031.1796347
GTATCAA5953700.031.0607939
GGTATCA5947150.031.0261968
AACGCAG5957850.030.9416614
ACGCAGA5974950.030.85082815
TATCAAC6010300.030.82538210
CGCAGAG5969550.030.75125516
GAGTACA5992850.030.32917420
GTACATG5912800.030.30995622
AGAGTAC6072300.029.96809819
AGTACAT6060650.029.93952621
Produced by FastQC (version 0.11.5)