FastQCFastQC Report
Tue 18 Jun 2019
HHJHYBGXB_n02_HTO_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJHYBGXB_n02_HTO_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17200518
Sequences flagged as poor quality0
Sequence length55
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAGGCGCGCCGCTCTCTACGTTTA300140417.44949774187033No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTTGTGCTCAAAACTCAAGGTTT189677711.027441150318845No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCGTTTCCGCTTAACGGCGGTTT10269695.970570188641993No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAACGACTATCATAACCAGGTTT8031094.669097756241992No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCATATCGGGGCGAGACATGGTTT7926074.608041455495701No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCAGTCTTGAACAAAGAAGGGTTT6662663.8735228787877203No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGCACATATTACAAAGCCTAGTTT6009933.494040121349834No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCACAAGACTAGGCCACGAGTTT4469532.5984856967679693No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAGAAAGCTGGGTTCACATGGTTT4296802.498064302482053No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAGAAGCAGAGAACGAGAGGTTT3555792.0672575093378No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATTGTTGGTATAAGAGATGGGTTT3091041.797062158244304No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGCCATTGTGGCTCCGGCCGGTT2763571.6066783570122714No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTCCCTAAAAATTACAAGCGTTT2556481.4862808201473934No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTGGTGGTGAACTTAACCCGTTT2248481.3072164454582125No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCCTCCACCAACTAACAGGTTT1958451.1385994305520333No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACGATTGCATTGGTAACTGGGTTT1514350.880409531852471No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGCCATTGTGGCTCAGGCCGGTT1493240.8681366456521832No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTGTGCTCAAAACTCAAGGTTTA1306810.7597503749596378No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGTTTCCGCTTAACGGCGGTTTA1161890.6754970984013388No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGGAGCTAGTGTAAGGGGGTTTA1136410.6606835910406884No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTGCACATATTACAAAGCCTAGTT1136340.6606428945918954No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATACTGGGATGAAGAAGAGGGTTT904410.5258039321839029No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGTTGTGCTCAAAACTCAAGGTT685450.39850544035941243No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG560600.3259204170479052No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAACGACTATCATAACCAGGTTTA487030.28314844936646677No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGGCGCGCCGCTCTCTACGTTTAA463630.26954420791280825No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCATATCGGGGCGAGACATGGTTTA436400.2537132893323329No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGAAGCAGAGAACGAGAGGTTTA391570.22765012076961869No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCACGCCTG346330.20134858729254548No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTTTCTAT313660.18235497326301453No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCCATTGTGGCTCCGGCCGGTTG310360.18043642639134472No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCAGTCTTGAACAAAGAAGGGTTTA304750.1771748967095061No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCGCGCCGCTCTCTACGTTTAAG271520.15785571108963115No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTATAAATCGACTGTACACGTTTA261860.15223960115619772No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGTCCCTAAAAATTACAAGCGTT243210.1413969044420639No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTAGGCGCGCCGCTCTCTACGTTT239280.13911208953125714No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAA235980.13719354265958736No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCACATATTACAAAGCCTAGTTTA228460.1328215813035398No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTCCACAAGACTAGGCCACGAGTT226460.13165882562373993No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAGTCACTCATAGACGTGTTTA214490.12469973288013769No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAGCTCACAAGAACTGCTAAC195630.11373494681962484No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACGCCTGT193420.11245010179344599No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTTCCTCCACCAACTAACAGGTT184620.10733397680232654No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGAGCTAGTGTAAGGGGGTTTAA182390.10603750421934967No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGCG204.6226167E-449.00327333
AAGCAGT16801000.048.9982381
AGCAGTG16829300.048.8866962
CAGTGGT16795100.048.8409464
GCAGTGG16823500.048.8278733
AGTGGTA16812100.048.7950445
GTGGTAT16819850.048.739636
TGGTATC16802050.048.7086077
GGTATCA16825350.048.664668
GTATCAA16828400.048.602759
TATCAAC16821700.048.59113310
ATCAACG16830900.048.57555811
TCAACGC16827200.048.54164512
CAACGCA16837000.048.52519613
AACGCAG16876900.048.43455514
ACGCAGA16887050.048.39502715
CGCAGAG16893100.048.38466316
CAGAGTA16860650.048.3418218
GCAGAGT16869650.048.33170717
AGAGTAC16827100.048.3275719
Produced by FastQC (version 0.11.5)