FastQCFastQC Report
Tue 18 Jun 2019
HHJHYBGXB_n02_HTO_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJHYBGXB_n02_HTO_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16646631
Sequences flagged as poor quality0
Sequence length55
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAGGCGCGCCGCTCTCTACGTTTA290321617.44026163612325No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTTGTGCTCAAAACTCAAGGTTT183250811.008281495517021No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCGTTTCCGCTTAACGGCGGTTT9927685.963777295237697No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAACGACTATCATAACCAGGTTT7758554.660732853392377No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCATATCGGGGCGAGACATGGTTT7623234.579443131766422No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCAGTCTTGAACAAAGAAGGGTTT6418293.855608981781359No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGCACATATTACAAAGCCTAGTTT5801763.485245753329908No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCACAAGACTAGGCCACGAGTTT4331152.60181774918901No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAGAAAGCTGGGTTCACATGGTTT4153612.4951655383002125No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAGAAGCAGAGAACGAGAGGTTT3448702.071710486043693No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATTGTTGGTATAAGAGATGGGTTT2981831.7912513348797126No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGCCATTGTGGCTCCGGCCGGTT2660881.5984495601542439No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTCCCTAAAAATTACAAGCGTTT2467681.482390040363122No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTGGTGGTGAACTTAACCCGTTT2184091.3120312452411542No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCCTCCACCAACTAACAGGTTT1885121.1324333434194582No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACGATTGCATTGGTAACTGGGTTT1465370.8802802200637474No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGCCATTGTGGCTCAGGCCGGTT1429260.8585881431504069No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTGTGCTCAAAACTCAAGGTTTA1272770.7645811335639024No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGTTTCCGCTTAACGGCGGTTTA1125410.6760587172263265No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGGAGCTAGTGTAAGGGGGTTTA1093480.6568776589088807No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTGCACATATTACAAAGCCTAGTT1092720.6564211100732634No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATACTGGGATGAAGAAGAGGGTTT866760.5206819325784299No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGTTGTGCTCAAAACTCAAGGTT667540.4010060654314978No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAACGACTATCATAACCAGGTTTA472620.283913303538716No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGGCGCGCCGCTCTCTACGTTTAA447190.268636939210102No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCATATCGGGGCGAGACATGGTTTA418440.2513661773364232No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG414570.249041382607688No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGAAGCAGAGAACGAGAGGTTTA379320.22786592674517744No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCACGCCTG334790.2011157693109194No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTTTCTAT303620.18239125982909093No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCCATTGTGGCTCCGGCCGGTTG297550.17874487636567424No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCAGTCTTGAACAAAGAAGGGTTTA290770.17467198017424668No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCGCGCCGCTCTCTACGTTTAAG261860.15730510275622736No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTATAAATCGACTGTACACGTTTA252660.15177845895665015No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGTCCCTAAAAATTACAAGCGTT236890.14230507061759223No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTAGGCGCGCCGCTCTCTACGTTT234380.14079725801575105No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAA225040.13518651311487592No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTCCACAAGACTAGGCCACGAGTT221210.13288574727222585No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCACATATTACAAAGCCTAGTTTA220680.132567364531598No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAGTCACTCATAGACGTGTTTA209610.12591737030754152No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAGCTCACAAGAACTGCTAAC188550.11326616178372668No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACGCCTGT182880.10986006718116118No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGAGCTAGTGTAAGGGGGTTTAA179510.10783563352848993No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTTCCTCCACCAACTAACAGGTT177720.1067603408761809No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTA204.6270035E-448.99384339
AAGCAGT16235750.048.9935461
AGCAGTG16260800.048.886582
CAGTGGT16226300.048.844174
GCAGTGG16257050.048.8263053
AGTGGTA16242900.048.7933165
GTGGTAT16250700.048.7381066
TGGTATC16234150.048.7037477
GGTATCA16256450.048.660818
GTATCAA16263400.048.5915159
TATCAAC16260700.048.58290510
ATCAACG16271500.048.561911
TCAACGC16268400.048.5234212
CAACGCA16277050.048.5085913
AACGCAG16312200.048.41831214
ACGCAGA16321650.048.38639515
CGCAGAG16327900.048.3776416
CAGAGTA16287800.048.34078618
GCAGAGT16297450.048.3360617
AGAGTAC16261150.048.32780519
Produced by FastQC (version 0.11.5)