FastQCFastQC Report
Tue 18 Jun 2019
HHJHYBGXB_n02_HTO_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJHYBGXB_n02_HTO_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16744905
Sequences flagged as poor quality0
Sequence length55
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAGGCGCGCCGCTCTCTACGTTTA287666117.179321112899714No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTTGTGCTCAAAACTCAAGGTTT181825010.858526817560326No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCGTTTCCGCTTAACGGCGGTTT9867355.892747674591167No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAACGACTATCATAACCAGGTTT7692974.594215374766235No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCATATCGGGGCGAGACATGGTTT7606374.542498150930089No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCAGTCTTGAACAAAGAAGGGTTT6383103.811965490398422No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGCACATATTACAAAGCCTAGTTT5743703.430117997086278No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCACAAGACTAGGCCACGAGTTT4282752.557643653397854No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAGAAAGCTGGGTTCACATGGTTT4138092.471253196121447No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAGAAGCAGAGAACGAGAGGTTT3423002.0442038936619826No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATTGTTGGTATAAGAGATGGGTTT2975781.7771256391123151No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGCCATTGTGGCTCCGGCCGGTT2882621.721490805710752No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTCCCTAAAAATTACAAGCGTTT2451841.464230462937831No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTGGTGGTGAACTTAACCCGTTT2156141.287639434204016No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCCTCCACCAACTAACAGGTTT1872981.118537250584581No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGCCATTGTGGCTCAGGCCGGTT1570890.938130135703965No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACGATTGCATTGGTAACTGGGTTT1460560.8722414370221868No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTGTGCTCAAAACTCAAGGTTTA1268010.7572512355250747No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGTTTCCGCTTAACGGCGGTTTA1119040.6682868609884618No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGGAGCTAGTGTAAGGGGGTTTA1091320.6517325717882544No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTGCACATATTACAAAGCCTAGTT1088020.649761823073944No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATACTGGGATGAAGAAGAGGGTTT872420.5210062404056637No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGTTGTGCTCAAAACTCAAGGTT658050.3929852095308991No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG556960.3326146072491901No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAACGACTATCATAACCAGGTTTA474470.2833518613572308No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGGCGCGCCGCTCTCTACGTTTAA444600.265513599509821No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCATATCGGGGCGAGACATGGTTTA415750.24828447817410731No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGAAGCAGAGAACGAGAGGTTTA374740.2237934464244497No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCACGCCTG368390.2200012481408524No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCCATTGTGGCTCCGGCCGGTTG323360.19310948613921666No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTTTCTAT305290.18231814393691695No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCAGTCTTGAACAAAGAAGGGTTTA288640.17237482087835077No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCGCGCCGCTCTCTACGTTTAAG259090.15472766193657114No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTATAAATCGACTGTACACGTTTA250340.1495021918607481No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTAGGCGCGCCGCTCTCTACGTTT235380.1405681310225409No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGTCCCTAAAAATTACAAGCGTT229170.13685954026015676No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAA224050.13380189377007512No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTCCACAAGACTAGGCCACGAGTT216740.12943638676958752No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCACATATTACAAAGCCTAGTTTA216650.12938263907737907No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAGTCACTCATAGACGTGTTTA206170.12312401891799325No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACGCCTGT199630.11921835328417808No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAGCTCACAAGAACTGCTAAC188910.11281640594556971No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGATAAAATTTGAAATCTGGTTAG180700.10791342202299745No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTTCCTCCACCAACTAACAGGTT177570.10604419672730302No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGAGCTAGTGTAAGGGGGTTTAA176810.10559032732643152No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCCATTGTGGCTCAGGCCGGTTG176740.10554852356582495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT16332450.048.9915771
AGCAGTG16357800.048.8851472
CAGTGGT16322350.048.8459554
GCAGTGG16354150.048.825223
AGTGGTA16340400.048.7918365
GTGGTAT16349900.048.737886
TGGTATC16334600.048.703777
GGTATCA16356400.048.658228
GTATCAA16362600.048.594139
TATCAAC16359000.048.58332410
ATCAACG16371050.048.56339311
TCAACGC16368350.048.5261812
CAACGCA16371350.048.51401513
AACGCAG16407250.048.42502214
ACGCAGA16416450.048.38862615
CGCAGAG16419850.048.3805716
CAGAGTA16380500.048.34023318
GCAGAGT16392450.048.33293517
AGAGTAC16347250.048.32733519
GAGTACA16334650.048.3215720
Produced by FastQC (version 0.11.5)