FastQCFastQC Report
Tue 18 Jun 2019
HHJHYBGXB_n02_HTO_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJHYBGXB_n02_HTO_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16275283
Sequences flagged as poor quality0
Sequence length55
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAGGCGCGCCGCTCTCTACGTTTA282360617.349043945963952No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTTGTGCTCAAAACTCAAGGTTT178431010.963311667145819No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCGTTTCCGCTTAACGGCGGTTT9707085.9643079631856475No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAACGACTATCATAACCAGGTTT7550004.638936232322351No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCATATCGGGGCGAGACATGGTTT7350714.516486748648242No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCAGTCTTGAACAAAGAAGGGTTT6268543.8515705072532382No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGCACATATTACAAAGCCTAGTTT5584133.431049401721617No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCACAAGACTAGGCCACGAGTTT4187882.5731534130620033No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAGAAAGCTGGGTTCACATGGTTT4033742.4784453824858224No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAGAAGCAGAGAACGAGAGGTTT3355172.0615125402120507No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATTGTTGGTATAAGAGATGGGTTT2913671.7902422956332005No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGCCATTGTGGCTCCGGCCGGTT2611081.604322333442681No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTCCCTAAAAATTACAAGCGTTT2392651.4701126855981552No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTGGTGGTGAACTTAACCCGTTT2125581.3060172287019525No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCCTCCACCAACTAACAGGTTT1849961.1366684069333848No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACGATTGCATTGGTAACTGGGTTT1420450.8727651617486467No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGCCATTGTGGCTCAGGCCGGTT1397480.858651735886866No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTGTGCTCAAAACTCAAGGTTTA1234030.7582233746718874No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGTTTCCGCTTAACGGCGGTTTA1091830.6708516220578161No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGGAGCTAGTGTAAGGGGGTTTA1061030.6519272199445011No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTGCACATATTACAAAGCCTAGTT1050710.6455863163792606No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATACTGGGATGAAGAAGAGGGTTT848700.5214655868042356No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGTTGTGCTCAAAACTCAAGGTT650430.39964282034297033No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAACGACTATCATAACCAGGTTTA467180.28704877205514645No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG452150.2778139095953047No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGGCGCGCCGCTCTCTACGTTTAA439450.27001066586676253No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCATATCGGGGCGAGACATGGTTTA407540.2504042479630001No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGAAGCAGAGAACGAGAGGTTTA366410.225132797998044No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCACGCCTG322570.19819624641857228No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCCATTGTGGCTCCGGCCGGTTG292440.17968351149408585No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTTTCTAT290490.1784853756460026No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCAGTCTTGAACAAAGAAGGGTTTA284420.17475579380094342No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCGCGCCGCTCTCTACGTTTAAG253190.15556718737241004No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTATAAATCGACTGTACACGTTTA248020.1523905913033893No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGTCCCTAAAAATTACAAGCGTT229160.14080246715218409No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTAGGCGCGCCGCTCTCTACGTTT228550.14042766568175805No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAA222450.1366796509774976No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTCCACAAGACTAGGCCACGAGTT214720.13193011758996756No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCACATATTACAAAGCCTAGTTTA207430.1274509328040563No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAGTCACTCATAGACGTGTTTA202470.12440336674944455No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAGCTCACAAGAACTGCTAAC183370.11266777972462906No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACGCCTGT176690.10856339640914385No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTTCCTCCACCAACTAACAGGTT175470.10781379346829177No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGAGCTAGTGTAAGGGGGTTTAA173890.10684299621702431No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT15894050.048.994931
CGTACTT150.00913834548.9938438
AGCAGTG15920100.048.894982
CAGTGGT15885500.048.847854
GCAGTGG15922700.048.8270453
AGTGGTA15905000.048.80215
GTGGTAT15906600.048.7523736
TGGTATC15886850.048.71977
GGTATCA15911600.048.6747678
GTATCAA15910600.048.6174939
TATCAAC15900750.048.6083510
ATCAACG15920750.048.5852711
TCAACGC15915000.048.54710412
CAACGCA15926800.048.5281313
AACGCAG15965200.048.44310814
ACGCAGA15974800.048.40803515
CGCAGAG15981700.048.39970416
CAGAGTA15942000.048.3598618
GCAGAGT15952000.048.3524317
AGAGTAC15909450.048.34470419
Produced by FastQC (version 0.11.5)