FastQCFastQC Report
Tue 18 Jun 2019
HHJHYBGXB_n02_Guide.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJHYBGXB_n02_Guide.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15997335
Sequences flagged as poor quality0
Sequence length55
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG489146930.576774193951678No Hit
AGCTTACTATCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2029321.268536290575899No Hit
AGCTTACTATCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1706621.0668151914053186No Hit
AGCTTACTATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1545440.9660609095202419No Hit
AGCTTACTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1145420.7160067598759419No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAGGGG607440.37971324598753475No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAGGG576690.360491294331212No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGG184120.11509417037275271No Hit
AGCTTACTATCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGG166670.10418610349786388No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAAGGG166490.104073584756461No Hit
AGCTTACTATCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGG163430.10216076615261228No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAACT7250.049.025841
TAGCTTA550.049.025841
CTTGTCG600.049.0258371
CATTGTC150.00911480149.0258371
AGCTTAT8600.049.0258371
ACCTACC150.00911480149.0258371
AGCCTAC2100.049.0258371
CGCGTAC150.00911480149.0258371
CTTAGCC301.2010969E-649.0258371
CACTTAC150.00911480149.0258371
CGCTTAC1750.049.0258371
ATTACCT204.6121253E-449.0258331
AGCTCAC1150.049.0258331
ATTTGTC204.6121253E-449.0258331
AGCTTAC8785500.049.013561
TCTTACT24000.049.0117342
TCTTAAT1250.049.0117342
ACTTAAT3950.049.0117342
TCAATTC750.049.0117342
GCTTCTA5200.049.0117342
Produced by FastQC (version 0.11.5)