FastQCFastQC Report
Tue 18 Jun 2019
HHJHYBGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJHYBGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41636008
Sequences flagged as poor quality0
Sequence length28
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGG539950.12968342209944816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGAC43750.016.7316441
GCCGATG77400.016.593331
TGCGACG56150.016.4529151
GACGTTA70450.016.2429521
TGACTCC72250.016.1281931
CGGAGAA57650.015.3364081
CTGGACG36800.015.2182411
CCCGGAA35800.015.2122161
AAACGAG33700.015.14176759
GGGACCT38800.015.1157081
GGGATCC42550.014.9494371
AGCCGAA33050.014.9402078
TGGAGGA56000.014.6661751
AATCGAC63850.014.3824431
GTACGAC38100.014.2588228
CTCGAGG56850.014.214191
AGCCGGA23900.014.17211158
TACCGAA57300.014.1410391
CCGATGA31250.014.0875852
GAAGAAT75950.014.0651581
Produced by FastQC (version 0.11.5)