FastQCFastQC Report
Tue 18 Jun 2019
HHJHYBGXB_n01_RNA_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJHYBGXB_n01_RNA_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86246981
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGATG233950.017.2367821
TGCGACG185000.016.8313881
TCGGGAC136700.016.8061121
TGACTCC242650.016.6051711
AAACGAA182350.016.3398531
CTGGACG130650.016.0768811
AGTAAAC183900.015.9636188
CGGAGAA194950.015.8651021
GGGATCC144000.015.7019871
AGCCGAA99200.015.7005958
AATCGAC204700.015.56094551
TAGCCGA103050.015.487637
CCCGAAG103800.015.4442721
CCCGGAA116750.015.3615831
ATACCGA182850.015.2782291
GTTGAAC187350.015.2695041
GTACGAC127350.015.1753018
GACGTTA194750.015.0395861
GAAACCT198050.014.9778781
TAAGCAC237600.014.897381
Produced by FastQC (version 0.11.5)