FastQCFastQC Report
Tue 18 Jun 2019
HHJHYBGXB_n01_RNA_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJHYBGXB_n01_RNA_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92778672
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGATG253000.017.4003981
TGCGACG194800.016.7304341
TGACTCC265950.016.482781
TCGGGAC144100.016.4243261
AAACGAA193900.016.371151
GGGATCC151250.016.0334641
AGTAAAC204550.016.0080728
GTACGAC139900.015.8737058
CTGGACG146600.015.8064951
CGGAGAA210450.015.6849611
AGCCGAA106950.015.570588
CCCGAAG114300.015.5274191
ATACCGA198450.015.28056051
GTTGAAC201750.015.2105921
AATCGAC217250.015.2092111
GAAACCT217450.015.1193221
GACGTTA213150.015.1146081
TAGCCGA112150.015.0645477
CCCGGAA122100.015.0221141
GGATAGA123850.014.9680244