FastQCFastQC Report
Tue 18 Jun 2019
HHJHYBGXB_n01_HTO_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJHYBGXB_n01_HTO_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17200518
Sequences flagged as poor quality0
Sequence length28
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGAC38900.018.1313511
GCCGATG50150.018.0572071
TGCGACG49350.017.7479271
AGTGCCG34300.017.2587721
CGGGACG14850.017.1137072
AAACGAA39250.016.3156741
TTACGCC41500.016.2530211
TAAGCAC47100.016.0727161
TACCGAA49400.016.0148771
CGGAGAA45950.015.8044771
CGAAACA34250.015.70743
CGGACAC47700.015.6860021
AGACGGG19750.015.5379398
CATTCCG55250.015.474281
CTACAGA46350.015.4069511
CAATACG42750.015.3659681
CAACGGC49650.015.3358751
AACAACC64100.015.3290471
CGGAACC38500.015.3188371
CACCGAA39600.015.3042738
Produced by FastQC (version 0.11.5)