FastQCFastQC Report
Tue 18 Jun 2019
HHJHYBGXB_n01_HTO_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJHYBGXB_n01_HTO_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16646631
Sequences flagged as poor quality0
Sequence length28
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGATG50250.017.9981561
TGCGACG47450.017.668961
TCGGGAC37500.017.6040341
AAACGAA34200.016.8576661
AGTGCCG31550.016.7043041
TTACGCC40650.016.3210831
CACCGAA38300.016.3122548
GGCGGTC12750.016.221624
TACCGAA45350.016.1337991
AGTAACC38750.015.9572071
CGGAGAA45050.015.8260481
TAAGCAC46500.015.7821131
CCCGAAG38400.015.7015161
CGGGACG13050.015.6802482
CGAAACA32850.015.6063393
CTACAGA43200.015.3322181
CATTCCG54400.015.2902691
CCGGTCG11450.015.2770055
TAACGAC31800.015.2236171
TCCGGGA26600.015.2215361
Produced by FastQC (version 0.11.5)