FastQCFastQC Report
Tue 18 Jun 2019
HHJHYBGXB_n01_HTO_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJHYBGXB_n01_HTO_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16744905
Sequences flagged as poor quality0
Sequence length28
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGATG51550.018.2927911
TGCGACG49900.017.6407591
TCGGGAC34700.017.5991361
AGTGCCG30800.017.0052911
CGGAGAA49050.016.1518151
TACCGAA44850.016.069661
TAAGCAC47400.016.064071
TTACGCC38700.015.9506981
AACAACC62550.015.8850381
AAACGAA34950.015.8361141
CGGACAC48050.015.7322621
CCGGTCG12350.015.6787915
GGCGGTC10750.015.6584884
CACCGAA40900.015.5977278
AGTAACC39400.015.58352851
CCCGAAG37250.015.567261
CTACAGA43850.015.5076751
TAACCGT35150.015.4934453
CGGAACC36300.015.3684191
CGAAACA34650.015.367753
Produced by FastQC (version 0.11.5)