FastQCFastQC Report
Tue 18 Jun 2019
HHJHYBGXB_n01_HTO_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJHYBGXB_n01_HTO_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16275283
Sequences flagged as poor quality0
Sequence length28
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGAC37650.018.323651
GCCGATG50350.018.028681
TGCGACG46150.017.6667211
CCGGTCG10550.016.7889735
AGTGCCG33150.016.5957131
TTACGCC38050.016.3959961
TAAGCAC44950.016.1311451
CGAAACA30200.016.0285343
TACCGAA45150.016.010951
AAACGAA36050.015.901641
GGCGGTC10300.015.807894
CGGGACG12950.015.7164352
AGTAACC39400.015.6945751
CATTCCG50350.015.6685621
CGGAGAA43900.015.5896131
CGGACAC46600.015.583591
GTTGAAC47300.015.49253751
TAACGAC29600.015.4636161
CGGAACC34600.015.4550211
ACGGAAG33850.015.3423811
Produced by FastQC (version 0.11.5)